|Use of the fluorescence lifetime of fluorescent molecules for bioimaging
Co-sponsored：Leica Microsystems K.K.
Moderator: Shintaro Tanaka (Leica Microsystems K.K.)
Speaker：Yoshikatsu Sato (Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University)
Speaker：Suguru Osari (Leica Microsystems K.K.)
- In fluorescence imaging, target molecules are fluorescently labeled, and in most cases, images are obtained by using the brightness at the fluorescence wavelength of the fluorescent molecule. Analysis based on fluorescence brightness is simple, but it is easily perturbed not only by the conditions of excitation wavelength and fluorescence acquisition wavelength, which depend on the optical system of the microscope, but also by the concentration and photostability of fluorescent molecules. In addition, the influence of autofluorescent substances present in biological tissues or in chemical fixative solutions cannot be eliminated. On the other hand, imaging methods based on the fluorescence lifetime of fluorescent molecules have long attracted attention because they are not affected by the optical system, dye concentration, and thus can complement the weaknesses of the brightness-based analysis. The challenge of fluorescence lifetime imaging was the complexity of the system and the time required for the data acquisition, which may not have been sufficiently appealing to the average fluorescence imaging user. However, this was before Leica released FALCON. Leica's FALCON is a revolutionary fluorescence lifetime microscope system that removes the above shortcomings. This system enables fluorescence lifetime imaging with the ease of obtaining images with a confocal laser microscope. In this presentation, we will show an example of imaging using this system.
|Next Generation Platform to Accelerate Single Cell Analysis, GENESIS
Co-sponsored：Bio-Rad Laboratories K.K.
Moderator: Masazumi Mashiko (Bio-Rad Laboratories K.K.)
Speaker：Takashi Sugimoto（Bio-Rad Laboratories K.K.）
Speaker：Toru Yaegashi (Nihon Gene Research Laboratories Inc.)
- In many diseases, the cell is the fundamental unit for determining the onset, progression, and treatment of the disease. Obtaining information on the state of the cell, including its genome and proteins, is critical to understanding disease progression and response to therapy. This requires intact, viable cells that have not been damaged by harsh cell isolation and manipulation processes.
CelselectTM technology, one of the platforms of the GENESIS system, utilizes dedicated microfluidic slides with 56,400 microchambers that can process hundreds to millions of cells in a single experiment. It can be used for a variety of single-cell applications by capturing and separating immune cells, rare cells, CTCs, etc. based on cell size without the use of surface antigens such as EpCAM. In addition, CelsingleTM, a cell separation platform currently under development, uses a gentle gravity-based method to capture and separate individual cells while maintaining cell viability and structure for applications such as scRNA-Seq. As described above, the GENESIS system enables researchers to approach previously inaccessible information in a variety of single-cell applications.
In this seminar, we will introduce the technology and products of the GENESIS system, as well as the CTC measurement service using the GENSSIS B1system and its application provided by Nihon Gene Research Laboratories Inc.
|New Biomek NGeniuS & EMnetik 24 from Beckman Coulter Life Sciences, make the genomics workflow easier
Co-sponsored：Beckman Coulter K. K.
Speaker：Tomofumi Nakazaki（Beckman Coulter Life Sciences）
Speaker：Jun Onodera（Beckman Coulter Life Sciences）
- Even though innovations in technologies for various applications in next generation sequencing and synthetic biology have been made, you may still be bothered by troublesome sample prep. In order to solve those problem, Beckman Coulter Life Sciences introduces 2 new products:
First of all, we are introducing NGS library prep system Biomek NGeniuS which is suitable for various kits. The most remarkable feature of Biomek NGeniuS is the function which enables update of new optimized program for each kit via cloud server. It also enables to set the tube which is packed together with the kit, and due to these convenient features Biomek NGeniuS do not require proficiency for users.
The next one is the instrument which enable to realize Semi-automated DNA purification utilizing magnetic beads, called EMnetik 24. EMnetik 24 enables to combine sample and magnetic beads by automated stirring, and instantaneous collecting of magnetic beads by electromagnet contributes shortening prep time. EMnetik 24 with compact case body meets various applications in synthetic biology including DNA purification after enzyme reaction such as PCR and plasmid DNA from E. coli.
|Novel function of extracellular vesicles in lymphoma
Co-sponsored：FUJIFILM Wako Pure Chemical Corporation
Chairperson：Susumu Tasaki（FUJIFILM Wako Pure Chemical Corporation）
Speaker：Ai Kotani（Tokai University School of Medicine）
Speaker：Hana Onizuka（FUJIFILM Wako Pure Chemical Corporation）
- Since it was suggested that the extracellular vesicles which are the lipid bilayer vesicles released by cells work as intercellular communication tools, research has been accelerated worldwide. Especially in the field of cancer, extracellular vesicles are potential biomarkers or therapeutic targets, and their attention is increasing. At this luncheon seminar, Professor Ai Kotani at Tokai University School of Medicine will be invited to introduce the latest research on extracellular vesicles under the title of "Novel function of extracellular vesicles in lymphoma."
|2BT03||Dec.2 (Thu) 11:40-12:30 Room3（302）|
|Comprehensive and cutting-edge solutions for bioimaging: from high speed superresolution to large scale 3D imaging
Co-sponsored：Carl Zeiss Co., Ltd.
Chairperson：Akira Sato（Carl Zeiss Co., Ltd.）
Speaker：Rie Hayashi（Carl Zeiss Co., Ltd.）
Speaker：Erina Kuranaga（Graduate School of Life Sciences, Tohoku University）
- The field of bioimaging has continued to diversify and become more sophisticated with each passing day. We ZEISS provide four cutting-edge imaging solutions to meet a wide range of bioimaging requirements and to obtain imaging data that cannot be achieved with conventional techniques: LSM with Airyscan (Confocal and Super Resolution), Elyra (Super Resolution), Lightsheet and Lattice Lightsheet that is launched in late 2020.
In this seminar, we will introduce the features of the four latest technologies, and Prof. Erina Kuranaga of Tohoku University will give a lecture on how they are used in actual research sites.
|2BT15||Dec.2 (Thu) 11:40-12:30 Room15（501）|
|Frontiers in RNA Sequencing. The Spatial Transcriptome and Illumina's Latest Solutions
Chairperson：Reiko Fujiwara（Illumina K.K.）
Speaker：Takuya Yamamoto（Center for iPS Cell Research and Application (CiRA), Kyoto University, Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University）
Speaker：Kenichi Sajiki（Illumina K.K.）
- Dr. Takuya Yamamoto of Kyoto University will give a lecture on intracellular spatial transcriptomics in pluripotent stem cells, and Illumina will propose a simpler and more accurate RNA sequencing workflow that covers library preparation, sequencing, and data analysis.
■■Abstruct for Dr. Yamamoto's talk■■
Regulation of RNA localization to specific locations in cells is a universal phenomenon that is widely conserved in evolution. For example, in fertilized eggs of the African clawed frog and Drosophila, mRNAs important for development are localized to specific sites and cause local translation, resulting in cellular asymmetry. In mammals, local translation due to limited localization of mRNAs at specific synapses is thought to alter the efficiency of interneuronal signaling associated with learning and memory. However, due to technical limitations, to date RNA subcellular localization has been comprehensively investigated only in relatively large cell types such as neurons and Drosophila embryos. In addition, the RNA localization sites that can be analyzed are mainly intracellular organelles that can be biochemically isolated. In this seminar, I would like to review APEX-seq, which can comprehensively identify RNAs localized in specific locations, introduce its application to pluripotent stem cells, and discuss its future prospects.
|2BT16||Dec.2 (Thu) 11:40-12:30 Room16（502）|
|Luminescent Detection and Nucleic Acid Purification Technologies for Diagnosis, Treatment, and Warning of Viral Infections
Speaker：Yusuke Yasue (Promega KK, Technical Services, Senior Scientist & Consultant)
Speaker：Michiko Momoi (Promega KK, Technical Services, Senior Scientist & Consultant)
- The time has come to build a solid foundation for countermeasures against unpredictable viral infections by making more effective use of Japan's excellent science, technology and ideas. Promega is equipped with products that can support the diagnosis of viral infections, the development of vaccines and therapeutic agents, and the monitoring of infection status at various stages.
|2BT17||Dec. 2 (Thu) 11:40-12:30 Room17（503）|
|#WeMakeDNA: DNA Is Changing The World - How Synthetic Biology can Improve our Health, Food and Materials
Chairperson：Masanori Noguchi（Twist Bioscience）
Speaker：Osamu Ohara（Department of Applied Genomics, Kazusa DNA Research Institute）
Speaker：Chiho YoshidaChiho Yoshida (Application Specialist, Twist Bioscience)
- Title of Invited Presentation: Does High Throughput DNA Synthesis Help in Multi-omics Analysis?
Title of Company Presentation: Powering the Synthetic Biology and Genomics Evolution through High Throughput DNA Synthesis Technology
In order to make our seminars more attractive, we will be accepting pre-registration for luncheon webinar attendees. We will be offering Starbucks eGift (drink tickets) for 200 people who register.
Please register using the following form on our website:
We look forward to seeing you there!
For more information, please contact us at
|3BT02||Dec. 3 (Fri) 11:40-12:30 Room2（301）|
|Latest technology for molecular structure analysis using HPLC and MS
Chairperson：Jun Watanabe (Shimadzu Corporation)
Speaker：Hidenori Takahashi (Shimadzu Corporation)
- Chemical structural analysis of biopolymers is still a difficult process, in which HPLC and mass spectrometry (MS) are powerful analytical methods. In this seminar, we will introduce the latest technology related to structural analysis using HPLC and MS. For example, our original MS technology is a novel tandem mass stectrumetry using radical gas such as atomic hydrogen / oxygen. This method makes it possible to assign double bond positions and modified positions of hydroxyl groups in biopolymers. The seminar will also introduce an overview of these new technologies and examples of structural analysis of biological samples.
|3BT03||Dec. 3 (Fri) 11:40-12:30 Room3（302）|
|Long Read Sequencing
The Importance of Quality Control
Co-sponsored：Agilent Technologies Japan, Ltd
Speaker：Satoshi Namai (Application Specialist, Diagnostic and Genomics Group Japan, Agilent Technologies Japan, Ltd)
Speaker：Shinichi Morishita (Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo)
- In the long read sequencing workflow, it is necessary to check the quality of samples at various steps, such as starting sample genomic DNA and libraries.
The Agilent Femto Pulse System, a fully automated pulsed-field capillary electrophoresis system, can analyze high-molecular DNA in a short time and in minute quantities, making it a suitable solution for long read sequencing workflows.
In this presentation, Dr. Shinichi Morishita of the University of Tokyo will introduce how the Femto Pulse is used to handle ultra-trace DNA in long read sequencing workflows, and what new structural variations are found.
|3BT15||Dec. 3 (Fri) 11:40-12:30 Room15（501）|
|Frontiers in Bioimaging
Co-sponsored：NIKON SOLUTIONS CO., LTD.
Chairperson：Tsurumune Atsushi（NIKON SOLUTIONS CO., LTD.）
Speaker：Atsushi Miyawaki（Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science）
Speaker：Matsui Kumiko（NIKON CORPORATION）
|3BT17||Dec. 3 (Fri) 11:40-12:30 Room17（503）|
|Flexible and robust sequencing library preparation for SMART-Seq
Co-sponsored：TAKARA BIO INC.
Speaker：Ilona Holcomb (Takara Bio USA, Inc.)
- Takara’s family of SMART-Seq kits provide the greatest sensitivity among commercial and homebrew methods for generating cDNA for RNA-Seq. Here we introduce our PLUS sequencing library preparation module to add to the functionality and ease-of-use of Takara’s SMART-Seq products.