The Molecular Biology Society of JapanThe 45th Annual Meeting of the Molecular Biology Society of Japan Cooperated by the Biophysical Society of Japan

Introduction to latest breakthrough in DNBSEQ technology and newly released sequencer, G99.

Thank you for submitting your vote for this seminar theme via Twitter. Protein purification chromatography with the ÄKTA^™ system is widely used, and Superdex^™ and HiTrap^™ column resins are evolving. Let's make our separations better and our experiments more accurate!

Recent years, next-generation sequencing technology has been aggressively improved to be applied with higher accuracy, higher throughput, and lower cost, which contributes to diverse research fields. Moreover, an increased number of interest-specific sequencing kits and bioinformatic tools accelerate applications of sequencing technology in not only academic research, but also disease diagnostics, personalized medicine, preventive medicine, environmental evaluation, agriculture purpose and so on. BGI Group has been employing sequencing technology to support researchers throughout the world for more than twenty years. Based on knowledge and knowhow generated from our R&D of basic biology, equipment, sequencing chemistry and bioinformatic method, we provide optimized sequencing solutions for customers’ distinct interests. In this presentation, we will update our new progress in WGS, RNA-seq solutions and highly visualized bioinformatics data-mining system Dr.Tom.

Spatial multi-omics analysis is a powerful tool to visualize gene or protein expression within histopathological samples such as heterogeneous regions of tumors, the adjacent microenvironment and associated inflammatory/immune cells. In this presentation, we will focus on GeoMx® which enables whole transcriptome analysis of region of interest (ROI), and CosMx™ which will bring revolution to single-cell analysis. We will explain each technology and how to use them according to the resolution and the number of detection target plex.

Transcriptome analysis by NGS can analyze gene expression levels in a highly sensitive and comprehensive. However, there are some challenges such as the cost of analysis and the complexity of data analysis, as well as the handling of clinical samples with poor quality such as fragmented RNA. Here, we propose 3' transcriptome analysis that can handle fragmented RNA and Stranded RNA-seq that pursues cost performance and simplicity of the workflow. We will also introduce "RNA-seq Analysis Portal", an easy and free cloud-based analysis system. This analysis method takes the stress out of data analysis and fast-tracks your path to gene expression

Novel evaporation system can reduce the concentration time of water to 1/5.
We will announce the technical information and original application with metabolomic samples, protein and DNA as well as distilled water.

Biacore^™ 1 Series, a 1-needle SPR platform with 6 flow cells and innovative software, is a major evolutionary step forward. Introducing the new ways to fulfill future research needs with Biacore’s high quality, consistent data.

Single cell sequencing is now a common method for scientists. Live-seq enables us to capture transcriptome of living cells (Chen, Nature, 2022 Aug), however, Live-seq could not reach subcellular components. Okada et al established intra-single cell sequencing (iSCseq) (Okada, bioRxiv 2022 Sep). iSCseq is a combinatorial method of subcellular sampling under comfocal microscopy and making cDNA library only with commercially available kit. iSCseq provides us another view of cell. will be the next core technology in cellular biology. I will share the novel technique iSCseq.

In recent years, there has been an increasing need in the rapidly developing field of liquid biopsy to extract nucleic acids from large-volume samples, and to minimize the manual labor required for FFPE samples in pathology and biobanks.
The EZ2 connect (24 samples) was created to meet these needs.
This benchtop nucleic acid purification system is based on the concept of eliminating tedious sample pretreatment and manual dispensing of reagents to the maximum extent possible.
All protocols are pre-installed, and the easy-to-use touch panel and pre-packed reagent cartridges simplify nucleic acid purification.

In this presentation, Kiagen's marketing staff will introduce what can be done now and what advantages can be gained by using this device.

Setsuro Tech Inc. is a startup based on the technology and know-how cultivated Tokushima University. We produce and deliver genome-edited mice, genome-edited cultured cells, genome-edited iPS cells, utilizing our unique patented technologies (GEEP method, VIKING method, and ST8). Although now it has become easier to engage in genome editing tests, it is still faster and less time to outsource the work, considering the time-consuming process of breeding and cloning mice. In order to give you more time to concentrate on your research, we introduce you to our contract genome editing service.